Apoenzyme of Pseudomonas cepacia salicylate hydroxylase. Preparation, fluorescence property, and nature of flavin binding.
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چکیده
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متن کاملSalicylate Hydroxylase, a Monooxygenase Requiring Flavin Adenine Dinucleotide. I. Purification and General Properties.
Hydroxylation reactions play important roles in many biosynthetic and catabolic processes. The mechanism of enzymatic hydroxylation reactions, however, has not been fully understood, mainly because the enzymes that catalyze such reactions are particulate or unstable and the extensive purification has met with great difficulties. In a preliminary report (2), we described the isolation from a soi...
متن کاملQuantification of salicylate in serum by use of salicylate hydroxylase.
In this enzymatic method for salicylate in serum, Pseudomonas salicylate hydroxylase (EC 1.14.13.1) is used. This stable enzyme catalyzes the stoichiometric, unidirectional conversion of salicylate and NAD(P)H to catechol and NAD(P)+ in the presence of molecular oxygen. The concentration of salicylate in a clinical sample is determined by measuring the delta A at 340 nm as compared with a stand...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43578-2